《植物生理学报》 2018, 54(10): 1553-1560
通信作者:彭福田;E-mail: pft@sdau.edu.cn
摘 要:
为探讨桃SnRK1蛋白激酶对植株生长发育进程的调控作用, 本研究从毛桃[Prunus persica (L.) Batsch]叶片中克隆到SnRK1蛋白激酶α催化亚基编码基因PpSnRK1α, 并通过农杆菌介导转化拟南芥(Arabidopsis thaliana), 获得超表达PpSnRK1α的拟南芥株系4-1、4-2和4-3。研究发现, 在MS培养基中, 转基因株系幼苗的平均真叶数比野生型(WT)增多, 平均鲜重约是WT的2倍, 侧根数显著增加, 而主根的长度明显变短, 约是WT主根长度的1/2。在营养钵中, 超表达PpSnRK1α的拟南芥株系4-2和4-3的花期及衰老进程比WT延迟, 与衰老相关的基因SEN1和SEN5的表达量显著降低。上述结果表明, 桃PpSnRK1α基因促进拟南芥幼苗的生长, 延缓植株的花期及衰老进程。关键词:桃; PpSnRK1α; 超表达; 生长; 花期; 衰老
收稿:2018-07-06 修定:2018-09-25
资助:国家自然科学基金(31672099)、 国家现代农业产业技术体系(CARS-31-3-03)和山东省“双一流”建设奖补资金(SYL2017YSTD10)。
Corresponding author: PENG Fu-Tian; E-mail: pft@sdau.edu.cn
Abstract:
In order to research the effects of sucrose non-fermenting-1-related protein kinase 1 (SnRK1) of peach (Prunus persica) on plant growth and development processes, in this study, the catalyzed subunit coding gene of SnRK1 protein kinase, named PpSnRK1α, was cloned from peach leaves. The PpSnRK1α-overexpression Arabidopsis lines 4-1, 4-2 and 4-3 were obtained by agrobacterium-mediated transformation. The study showed that, in MS medium, transgenic Arabidopsis lines had more true leaves than WT, the average fresh weight were about twice as much as WT, and the number of lateral roots increased significantly, while the tap roots length were significantly shorter, about half of the WT. In nutritional medium, flowering and aging processes of 4-2 and 4-3 Arabidopsis lines were more delayed than WT, and expression level of genes related with senescence, SEN1 and SEN5, were significantly lower than WT. The above results showed that PpSnRK1α could promote the growth of Arabidopsis seedlings, delay the flowering and aging processes of the plants.Key words: peach; PpSnRK1α; overexpression; growth; flowing; aging
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